| SRSF1

Serine/arginine-rich splicing factor 1 (SRSF1) also known as alternative splicing factor 1 (ASF1), pre-mRNA-splicing factor SF2 (SF2) or ASF1/SF2 is a protein that is encoded by the SRSF1 gene.ASF/SF2 is an essential sequence specific splicing factor involved in pre-mRNA splicing.SRSF1 is the gene that codes for ASF/SF2[9] and is found on chromosome 17. The resulting splicing factor is a protein of approximately 33 kDa.ASF/SF2 is necessary for all splicing reactions to occur, and influences splice site selection in a concentration-dependent manner, resulting in alternative splicing.In addition to being involved in the splicing process, ASF/SF2 also mediates post-splicing activities, such as mRNA nuclear export and translation.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human SRSF1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	88-102	97
Plasma(n=8)	94-107	104
Cell Culture Supernates(n=8)	96-110	107

 

| Linearity

Samples were spiked with high concentrations of Human SRSF1 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	82-93	88-96	90-110
	Average (%)	91	93	97
1 : 4	Range (%)	89-103	87-110	105-115
	Average (%)	94	98	109